OMB Meeting Book_9-11-14

COMMENT PARAMETERS 

USER COMMENTS 

AOAC OMA 

2011.10 

Method Name/Manuscript Title 

Vitamin B12 in Infant Formula and Adult Nutritionals by HPLC 

Manuscript Reference  In your experience using the method, does the  method perform according to the method’s  applicability as written?  In your experience with the method, is there  any safety concerns identified while using or  regarding use of the method?  Do you have any information that supports  regarding the reproducibility of the method as  written:  If so, please specify and submit  information?  Please attach any documentation to support  your feedback, if available.    Based on your experience with the method,  are there any recommended changes to the  AOAC First Action Method as written? 

Potassium Cyanide is a licensed chemical, use is restricted and labs  are not getting KCN. Use and Disposal is binding to law and hence the  use is completely stopped.  Chemical which can replace KCN is  required. 

1. Use of potassium cyanide should be avoided.  2. Modification is required for the process of heating of sample in a 

100 ml volumetric flask in oven at 105 OC for 1 hr.   3. Injection of 2 ml sample on HPLC is little difficult.