OMB Meeting Book_9-11-14
AOAC INTERNATIONAL Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)
COMMENT PARAMETERS
USER COMMENTS
AOAC OMA
2012.10
Method Name/Manuscript Title
Simultaneous Determination of 13‐cis and all‐trans Vitamin A Palmitate (Retinyl Palmitate), Vitamin A Acetate (Retinyl Acetate), and Total Vitamin E (DL‐α‐Tocopherol and DL‐α‐Tocopherol Acetate) in Infant Formula and Adult Nutritionals by liquid chromatography Extraction Method appears to be simple for the prescribed matrices of Milk, Soyabased infant formula and the hydrolyzed protein adult forumalas.
Manuscript Reference In your experience using the method, does the method perform according to the method’s applicability as written? In your experience with the method, is there any safety concerns identified while using or regarding use of the method? Do you have any information that supports regarding the reproducibility of the method as written: If so, please specify and submit information? Please attach any documentation to support your feedback, if available. Based on your experience with the method, are there any recommended changes to the AOAC First Action Method as written?
None
Though peaks are sharp, Baseline drift is found to be more in Vitamin E chromatograms. Optimization is required for LC conditions ‐ could be change of column, mobile phases, pH, mobile phase composition ‐ to avoid the quantification at low concentrations. Also, the pH of the sample extract at the time of addition of acidified methanol can be specified.
Chromatographic conditions need improvement (as stated above).
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