OMB Meeting Book_9-11-14

AOAC INTERNATIONAL Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)

COMMENT PARAMETERS 

USER COMMENTS 

AOAC OMA 

2012.10 

Method Name/Manuscript Title 

Simultaneous Determination of 13‐cis and all‐trans Vitamin A  Palmitate (Retinyl Palmitate), Vitamin A Acetate (Retinyl Acetate),  and Total Vitamin E (DL‐α‐Tocopherol and DL‐α‐Tocopherol Acetate)  in Infant Formula and Adult Nutritionals by liquid chromatography  Extraction Method appears to be simple for the prescribed matrices  of Milk, Soyabased infant formula and the hydrolyzed protein adult  forumalas. 

Manuscript Reference  In your experience using the method, does the  method perform according to the method’s  applicability as written?  In your experience with the method, is there  any safety concerns identified while using or  regarding use of the method?  Do you have any information that supports  regarding the reproducibility of the method as  written:  If so, please specify and submit  information?  Please attach any documentation to support  your feedback, if available.    Based on your experience with the method,  are there any recommended changes to the  AOAC First Action Method as written? 

None 

Though peaks are sharp,   Baseline drift is found to be more in   Vitamin E chromatograms.  Optimization is required for LC conditions  ‐ could be change of column, mobile phases, pH, mobile phase  composition ‐  to avoid the quantification at low concentrations.   Also, the pH of the sample extract at the time of addition of acidified  methanol can be specified. 

Chromatographic conditions need improvement (as stated above).