OMB Meeting Book_9-11-14

AOAC INTERNATIONAL Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)

COMMENT PARAMETERS 

USER COMMENTS 

AOAC OMA 

2012.13 

Method Name/Manuscript Title 

Labeled Fatty Acids Content in Milk Products and Infant Formula by  GC 

Manuscript Reference  In your experience using the method, does the  method perform according to the method’s  applicability as written? 

Sample preparation is found to be bit complicated with the use of  sodium ethoxide.   Chromatogram reveals a poor resolution of trans fatty acids.  Method recommends the cold fat extraction for product  supplemented with PUFA having fish oil. Profile may change due to  incomplete fat extraction.    Naturally occurring trans‐fatty acid‐ Vaccenic acid found in the fat of  ruminants and in dairy products such as milk, butter may interfere.   As DHA and EPA content is very low in health drinks the recovery may  affect. 

In your experience with the method, is there  any safety concerns identified while using or  regarding use of the method?  Do you have any information that supports  regarding the reproducibility of the method as  written:  If so, please specify and submit  information?  ‐  Please attach any documentation to support  your feedback, if available.    Based on your experience with the method,  are there any recommended changes to the  AOAC First Action Method as written? 

None  

Need simple sample preparation procedure.