OMB Meeting Book_9-11-14

COMMENT PARAMETERS 

USER COMMENTS 

AOAC OMA 

2012.13 

Method Name/Manuscript Title 

Determination of labeled fatty acids content in milk products and  infant formula by capillary gas chromatography GC/FID 

Manuscript Reference  In your experience using the method, does  the method perform according to the  method’s applicability as written?  In your experience with the method, is there  any safety concerns identified while using or  regarding use of the method?  Do you have any information that supports  regarding the reproducibility of the method  as written:  If so, please specify and submit  information?  Please attach any documentation to support  your feedback, if available.    Based on your experience with the method,  are there any recommended changes to the  AOAC First Action Method as written? 

Yes, for the fatty acids targeted. 

No, not if the proper personal protective equipment is used. 

The qualification sample done prior to the collaborative study was  done in triplicate.  That data has been provided already to the study  coordinator. 

Since the fatty acid distribution in natural samples is not even (that is,  some are very high level while others are very low), consider the use  of multiple calibration standards of different concentrations for more  accurate quantitation.