OMB Meeting Book_9-11-14

AOAC INTERNATIONAL Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)

COMMENT PARAMETERS 

USER COMMENTS 

AOAC OMA 

2012.22 

Method Name/Manuscript Title 

Vitamin C in Infant Formula and Adult Nutritionals by UPLC‐UV 

Manuscript Reference  In your experience using the method, does the  method perform according to the method’s  applicability as written?  In your experience with the method, is there  any safety concerns identified while using or  regarding use of the method?  Do you have any information that supports  regarding the reproducibility of the method as  written:  If so, please specify and submit  information?  Please attach any documentation to support  your feedback, if available.    Based on your experience with the method,  are there any recommended changes to the  AOAC First Action Method as written? 

As Vitamin C is unstable and oxidized to dehydroascorbic acid,  method suggests use TCEP as a reducing agent.  Dehydroascorbic acid  which has very poor response on UV may show decrease in the peak  area of ascorbic acid after keeping for long time.   LC column with 1.7 µm particle size may create problem of  backpressure after few injections due to sample matrix. 

Even though after using the TCEP as a reducing agent need to check  stability of the standards and sample extract. And should be  mentioned in the method. (Watchful for the response of standards  and sample extract.)