OMB Meeting Book_9-11-14

12 ( 2 ) At each working standard concentration, the peak areas of standards injected at the beginning and end of a set of samples should not increase or decrease by more than 10%. ( e ) Calculation of vitamin B concentrations in samples.— The vitamin B concentration in each injected sample preparation is

12

extrapolated from the vitamin B The concentration of vitamin B

standard curve prepared above. in each product can then be 12

12

calculated.

C = C × D  ss × D  V p i 1 2

p where C = product concentration in  g/kg; C = vitamin B concentration of the injected sample preparation extrapolated from standard curve in  g/L; D = volume of the first dilution in mL (100 mL); ss = sample size in g; D = volume of the second (final) dilution in mL; V = volume of filtrate loaded onto the cartridge in mL. For each set of samples, the control result must be within 3 standard deviations of the control mean. i 12 1 2

G. Validation Data Note: SLV and MLT data from the ERP checklist will be added to this section. Data tables will be added by September 19.

References: J. AOAC Int . 95 , 313(2012); AOAC SMPR 2011.005 J. AOAC Int . 95 , 293(2012)

© 2012 AOAC INTERNATIONAL