OMB Meeting Book - Jan. 11, 2018

2016.03 (Nov. 2017) - Chlor-02/04 Final Action Review FOR ERP USE ONLY DO NOT DISTRIBUTE

protein). In addition, IDF (International Dairy Federation) matrixes were added to this MLT in planning to be able to expand the scope to include dairy. All samples were blinded and codified before sending to participating laboratories.

Method Chloride in Milk, Milk Powder, Whey Powder, Infant Formula and Adult Nutritionals Potentiometric titration. Caution: Refer to Material Safety Data Sheets prior to use of chemicals. Use appropriate personal protective equipment when performing testing. A. Principle This document specifies one method (combined [6-9]) for the determination of chloride in milk, milk products, infant formula and adult nutritionals by potentiometry, with an analytical range of 0.35 mg chloride/100 g to 1060 mg chloride/100 g reconstituted product, or ready-to-feed liquids. Reconstitute powder samples by dissolving 25 g powder sample in 200 g warm water (40 ° C), while ready-to-feed products (RTF) are used as-is. Butter and cheese are prepared for liberation of chloride. Precipitate proteins by adding precipitation reagents I and II and centrifuge. Acidify the supernatant with nitric acid solution. Titrate chloride ions against standardized silver nitrate solution, 0,1 mol/l, potentiometrically using a silver electrode to detect the end point.

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