OMB Meeting Book - Jan. 11, 2018

2016.03 (Nov. 2017) - Chlor-02/04 Final Action Review FOR ERP USE ONLY DO NOT DISTRIBUTE

(c). Milk, milk powders, infant formula and adult nutritional products

For high protein samples requiring additional protein precipitation than that accomplished by addition of nitric acid solution, proceed with (F. d-f), Otherwise proceed with (g). (d).Weigh an appropriate (well mixed) aliquot of RTF or reconstituted (E. a) powder (e.g. 25 g accurate to 0.1 mg) into a 50 ml centrifuge tube. For samples with a high chloride content, weigh a smaller test portion e.g. 5 g of reconstituted or RTF product. (e). Transfer 2.5 ml precipitating solution I (D. c) and 2,5 ml precipitating solution II (D. d) into the tube. Complete to 50 ml with water. Mix well. If foam impacts the constant volume, add one or two drops of defoaming agent (C. l).

(f). Centrifuge at 12000 g for 5 min at 4 °C. Then equilibrate to room temperature.

(g).Accurately transfer either 10 ml supernatant from steps (D. d-f) or weigh an appropriate aliquot of RTF or reconstituted (E. a) powder (e,g, 25 g accurate to 0.1 mg). For samples with a high chloride content, weigh a smaller test portion e.g. 5 g of reconstituted or RTF product into a 120 ml sample beaker or autosampler cup. Add 5 ml nitric acid solution (D. e), and 50 ml of water before titration. Add a magnetic stirring rod (if the

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