OMB Meeting Book - Jan. 11, 2018

DQG QJ P/ JOXWHQ FDOLEUDWHG WR WKH :*3$7 JOLDGLQ KLJKO\ SXUL¿HG JOLDGLQ IURP GLIIHUHQW (XURSHDQ ZKHDW varieties). ( c ) Conjugate solution (peroxidase-labeled antibody, ready-to- use) ²2QH ERWWOH FRQWDLQLQJ P/ ( d ) Substrate solution (stabilized peroxide substrate and 3,3 ƍ ,5,5 ƍ -tetramethyl-benzidine in a dilute buffer solution) ²2QH ERWWOH FRQWDLQLQJ P/ ( e ) Stop solution (1 N H 2 SO 4 ) ²2QH ERWWOH FRQWDLQLQJ P/ ( f ) Diluent buffer ²2QH ERWWOH FRQWDLQLQJ P/ RI î FRQFHQWUDWHG GLOXHQW EXIIHU &RQWDLQV D ¿QDO FRQFHQWUDWLRQ RI P0 3%6 7ZHHQ VRGLXP FKORULGH 7ZHHQ ZLWK ¿VK JHODWLQ 6LJPD DQG 3URFOLQ DV D SUHVHUYDWLYH ( g ) Wash buffer ²2QH ERWWOH FRQWDLQLQJ P/ RI î FRQFHQWUDWHG ZDVK EXIIHU &RQWDLQV D ¿QDO FRQFHQWUDWLRQ RI P0 3%6 7ZHHQ VRGLXP FKORULGH 7ZHHQ ZLWK Proclin as preservative. ( h ) Extraction solution ²2QH ERWWOH FRQWDLQLQJ P/ RI ready-to-use proprietary extraction solution containing reducing agents. ( i ) Fish gelatin ²2QH VDFKHW FRQWDLQLQJ J Additional reagents needed, but not provided with the test kit: ( j ) Distilled or deionized water . Due to the sensitivity of the assay, a gluten-free environment must be maintained. It is preferable to perform the assay in a separate room from that used for sample preparation and extraction. Make sure balance and the surrounding space, as well as equipment VXFK DV VSDWXODV DUH FOHDQ &OHDQLQJ FDQ EH GRQH E\ XVLQJ D alcoholic solution. Spatula should be cleaned after each sample ZHLJKLQJ E\ D DOFRKROLF VROXWLRQ 6WRUH NLW DW ± ƒ& ± ƒ) DQG OHW DOO FRPSRQHQWV HTXLOLEUDWH WR ± ƒ& ± ƒ) EHIRUH XVH Include ready-to-use standards in duplicates to each run of samples. Use separate pipet tips for each standard and each sample extract to avoid cross-contamination. It is recommended that an eight-channel pipettor is used to SHUIRUP WKH DVVD\ 1R PRUH WKDQ VDPSOHV DQG VWDQGDUGV WRWDO should be run in one experiment when using an eight-channel SLSHWWRU ZKHQ VDPSOHV DQG VWDQGDUGV DUH DGGHG LQ GXSOLFDWH e.g., six test strips). If using only single-channel pipets, it is recommended that no more than a total of 16 samples and standards are analyzed in one experiment (eight when standards and samples are added in duplicate, e.g., two test strips). E. Preparation of Components Delivered with the Kit ( a ) Sample dilution buffer .—Dilute diluent buffer concentrate ZLWK GLVWLOOHG ZDWHU H J DGG P/ RI FRQFHQWUDWHG GLOXHQW EXIIHU WR P/ GLVWLOOHG ZDWHU 'LOXWLRQ EXIIHU PD\ EH XVHG ZLWKLQ K LI VWRUHG DW ƒ& ( b ) Wash buffer .—If a precipitate is formed during storage of the wash buffer concentrate, the concentrate should be warmed XS XQWLO LW LV GLVVROYHG 'LOXWH ZDVK EXIIHU FRQFHQWUDWH ZLWK GLVWLOOHG ZDWHU H J DGG P/ RI FRQFHQWUDWHG ZDVK EXIIHU WR P/ GLVWLOOHG ZDWHU :DVK EXIIHU PD\ EH XVHG ZLWKLQ ZHHN LI VWRUHG DW ƒ& ( k ) Ethanol ² Y Y D. General Instructions

7DEOH % &URVV UHDFWLYLW\ RI WKH * DQWLERG\ * DQWLERG\ VKRZV QR FURVV UHDFWLYLW\ WR YDULRXV QXWV RLOV VHHGV VWDUFKHV RU JOXWHQ IUHH JUDLQV

Romer extraction solution, mg/kg gluten Gluten, %

Food category

Food sample

Gluten-containing grains

:KHDW ÀRXU

72222

7.2

Barley (Cumion) 'XUXP ZKHDW Spelt (Ostro) Rye (Capitan)

292390 15733 81926 41577

29.2

1.6 8.2 4.2

Naturally gluten-free grains

Soya bean

<4

Soya mince %XFNZKHDW

<4 <4 <4 <4 <4 <4 <4

5LFH ÀRXU

Quinoa

Corn kernels

7HII ÀRXU

Millet

Oats

Bastion

4.3 7.4

00-61 Cn

%UDFKDQ

<4

Husky

6.3 6.6

Fusion Pecan Walnut Almond &DVKHZ

Nuts

<4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4 <4

Macadamia

Peanut

Hazelnut Pine nut 3LVWDFKLR

Seeds

Golden linseed Brown linseed

Poppy

Sesame Mustard

Oils

Hazelnut oil

Walnut oil

Vegetable oil 6XQÀRZHU RLO 7DSLRFD VWDUFK :KHDW VWDUFK 3RWDWR VWDUFK

6WDUFKHV

Miscellaneous

$PDUDQWK

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