PAC_App_CVs

2000 1999

Mega-Surgery laboratory training course through RARC. The plant cell wall and fiber in animal and human nutrition (875) Statistical methods for bioscience II (572) Seminar in animal nutrition (931)

1998

Comparative animal nutrition (311) Seminar in animal nutrition (931) Statistical methods for bioscience I (571)

1997

PROFESSIONAL SKILLS:

Administration and Management : Administrate; Industry, Federal and State sponsored research grants, fee-for-service contracts, consulting services, and a University of Wisconsin-Madison Foundation account. Manage large breeding colonies of Wisconsin Miniature Swine TM , Wisconsin Miniature Swine – Familial Hypercholesterolemia TM and Rapacz Familial Hypercholesterolemic Swine for agricultural and translational bio-medical research. Establish and maintain collaborative relationships. Hire full time, limited time and student hourly employees. Supervise and train; undergraduate, graduate, international scholars and professional personnel. Oversight over multiple Principal Investigator research program’s annual operational budget at the University of Wisconsin-Madison. Manuscript preparation, grantsmanship, continuation applications, animal care and bio-safety protocols. Principal Investigator: Research emphasis in the fields of phytochemistry, mucosal immunology and cardiovascular disease. Responsible for generating operating funds through competitive intra- and extramural grants, sponsored research agreements and business service agreements with industry. Medical Imaging Instrumentation : Phased and linear array echocardiography ultrasound (GE, Siemens, Ultrasonix systems), C-arm fluoroscopy/x-ray, Optical Coherence Tomography, and Intravascular Ultrasound. Chemistry Instrumentation: Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (Bruker: Reflex II, Biflex II & Ultraflex), electrospray ionization mass spectrometry (Shimadzu LCMS 2010), high performance liquid chromatography (Waters, photo diode array detector / Empower & Millennium 32 software), counter current chromatography (Pharama-Tech). Repair, maintain and trouble-shoot instruments. Surgical (Large Animals) : Contrast enhanced ultrasound (CEUS) quantification of atherosclerosis and vasa vasorum, high intensity focused ultrasound (HIFU) arterial repair, transesophageal ultrasound identification of patent foramen ovale, radio- frequency ablation liver resection, dual energy x-ray (DEXA) analysis of body composition, subcutaneous placement of vascular access ports, Cesarean section, peripheral artery angioplasty to induce vascular lesions, implantation of intracranial pressure transducer. Surgical preparation, record keeping, anesthesia. Technical : Analytic, semi-preparative and preparative liquid chromatography. Folin Ciocaleau, Butanol-HCl, DMAC assays for quantification of polyphenols from foods/forages. Crude fiber (NDF, ADF and lignin) and protein (Kjeldahl) forage analysis. Histological techniques from tissue preparation, cryostat, microtome, histo-chemistry, and microscopy. Quality control, experimental design, record keeping, data analysis and results published.

TEACHING / MENTORING EXPERIENCE:

Teaching Assistant 1999 “The plant cell wall and fiber in animal and human nutrition”, in the Dept. of Dairy Science at the University of Wisconsin-Madison. Mentor Biology 152 Undergraduate Independent Research Project 2009 Mike Shea – ‘Quantification of total polyphenols in pomegranates by the use of a pomegranate standard and method’. 2008 Michelle Wenzel – ‘Ultrasound guided transcutaneous intra-arterial injection to generate atherosclerotic lesions’. 2007 Rajeev Chaudhary – ‘The Effects of Familial Hypercholesterolemia (FH) on FH Cross Swine and Their Relationship with FH Cross Humans’. 2007 Jake Jursada- ‘ The effect of diet composition (fructose and fat) on uric acid levels in urine’. 2006 Allison Donenberg – ‘The effect of cranberry-filled diet on the amount of polyphenols, particularly cranberry flavonoids, in a sow’ urine’. 2006 Laura Nicholl- ‘ The effects of a cranberry powder diet on the levels of polyphenolic compounds in sow urine’.