RI-ERP-FINALACTION-Recommendations

OMAMAN-20 C/ In House Validation Report ERP Use Only - March 2015

3.1.3

Test Kit reagents

3.1.4 The test kit consists of dip-sticks (25 x), sample diluent, test tubes (30 x), disposable pipettes (25 x), evaluation card (1 x). 3.1.5 Reagent one: 25 x dip-sticks in a tube 3.1.6 Reagent two: 30 x empty test tubes 3.1.7 Reagent three: 25 x disposable pipettes 3.1.8 Reagent four: sample diluent (60 mL), ready to use, transparent capped bottle 3.1.9 Reagent five: 1 x evaluation card

3.2

Additional Supplies and Reagents

3.2.1 3.2.2 3.2.3 3.2.4 3.2.5 3.2.6 3.2.7 3.2.8 3.2.9

Variable 20 – 200 µL and 200 – 1000 µL micropipettes

Graduated pipettes

Graduated cylinders (plastic or glass ware)

Distilled water

Ethanol; 99% reagent grade

Cocktail (patented); R7006 (R-Biopharm AG, Germany); ready to use

Skim milk powder (food quality)

Centrifugal glass vials with a screw top

Paper filter

3.3

Apparatus

3.3.1 3.3.2 3.3.3 3.3.4 3.3.5

Scale

Laboratory mincer/grinder, pestle and mortar, or Ultra-Turrax Shaker (e.g. Roto Shaker Genie, Scientific Industries Inc.) Centrifuge (e.g. Minifuge RF, Kendro, Hanau, Germany)

Temperature controlled water bath 50 °C (e.g. GFL, Burgwedel, Germany)

3.4

(Standard) Reference Material

Not existing until today

3.5

Standard Solution and Spike Solution

AOAC Research Institute Expert Review Panel Use Only

The starting material used for preparation of in-house control solutions and spike solutions is identical. The preparation of the so-called “WGPAT Gliadin” is described elsewhere (3). It is stored at -20°C in a lyophilized form until reconstitution. For spiking purposes, the material is reconstituted in 60% aqueous ethanol resulting in a concentration of 1 mg prolamins/mL. The spike solution is diluted appropriately to the desired concentration. The solution is stable for a maximum of 4 weeks at 2-8°C.

3.6

General Preparation

3.6.1 Sample diluent -- The sample diluent is ready to use. Bring the solution to room temperature (20-25 °C) before use. Make sure that the buffer is not contaminated with gliadin during use.

3.6.2 60% aqueous ethanol -- Add 150 mL ethanol to 100 mL distilled water and shake well.

3.6.3 80% aqueous ethanol -- Add 200 mL ethanol to 50 mL distilled water and shake well.

3.6.4

Cocktail (patented) -- The Cocktail is ready to use (see 3.2.6).

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RIDA®QUICK Gliadin Validation report 2015-01-14