RI-ERP-FINALACTION-Recommendations

OMAMAN-20 C/ In House Validation Report ERP Use Only - March 2015

Table 5. Comparison of three different incubation temperatures (16°C, 23°C, 30°C) for the dip- stick procedure (10 repeats for each condition and concentration).

mg gliadin/kg incubation time

result for each repeat

POD

- - - - - - - - -

- - - - - - - - -

- -

- -

-

16°C

0,00

- - -

- -

-

0,50

23°C

0,10

+

- - -

- - -

- - -

30°C

0,00

16°C

0,20

-

+ +

+

+

- - -

1,00

23°C

0,40

+

+

-

- - -

- -

- -

+

30°C

0,20

-

+ + + + + + +

+ + + + + +

+ + + + + +

+ + + + + +

+ + + + + +

16°C

1,00

2,00

23°C

1,00

30°C

1,00

Spiked rice samples were also used for variation of the incubation time (Table 6). The number of positive results for the sample with a gliadin concentration of 1 mg/kg rises when the incubation time was increased from 4 min up to 6 min. This effect is only visible in a very small concentration range because at 0.5 mg/kg no sample was positive (except one outlier) while at 2 mg/kg all samples were positive irrespective of their incubation time. Nevertheless, for best reproducibility, the test kit manual strongly emphasizes that the readout should be performed exactly after 5 min.

Table 6. Comparison of three different incubation times (4 min, 5 min, 6 min) for the dip-stick procedure.

mg gliadin/kg incubation time

result for each repeat

POD

- - - - - - - - -

- - - - - -

- -

- -

-

- - - AOAC Research Institute Expert Review Panel Use Only - - - + - - - - - - - - - - + 4min 0,00 6min 0,00 0,10 5min 4min 0,20

0,50

- -

+ +

- - -

+

1,00

5min

0,40

+

+

-

-

-

-

- -

+ + + + + + + +

+ + + + + + + +

6min

0,60

+ + + + + + +

+ + + + + + +

+ + + + + +

4min

1,00

2,00

5min

1,00

6min

1,00

Page 17 of 27

RIDA®QUICK Gliadin Validation report 2015-01-14