RI-ERP-FINALACTION-Recommendations

OMAMAN-20 C/ In House Validation Report ERP Use Only - March 2015

Table 13. Results for soy flour spiked with WGPAT Gliadin (ethanol extraction); 20 replicates and 20 separate extractions per concentration

mg gliadin/kg

result for each repeat

POD

- - - - - - - -

- - - - - - - -

- - - - - - - -

- - - - -

-

-

0,00

0,00

- -

-

-

- -

0,50

0,00

- -

-

+ + + + + + + + + + + + + + + +

+ + + + + + + + + + + + + + + +

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

1,00

1,00

2,00

1,00

4,00

1,00

8,00

1,00

In case of cookies (Cocktail extraction) table 14 shows the results of 20 repeats and one extraction per repeat (also 20 separate extractions in total). Again, no false-positives were observed and the cut-off is at 4 mg gliadin per kg cookie.

Table 14. Results for cookies spiked with WGPAT Gliadin (cocktail extraction); 20 replicates and 20 separate extractions per concentration

mg gliadin/kg

result for each repeat

POD

- - - - - - - - - - - -

- - - - - - - - - - - -

- - - - - - - - - - - -

- - - - - - - -

-

-

0,00

0,00

- -

- AOAC Research Institute Expert Review Panel Use Only - - - - - - - - 0,00 0,00

1,00

2,00

- -

-

+ + + + + + + + + + + +

+ + + + + + + + + + + +

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

4,00

1,00

8,00

1,00

16,00

1,00

Page 23 of 27

RIDA®QUICK Gliadin Validation report 2015-01-14

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