RI-ERP-FINALACTION-Recommendations

OMAMAN-20 C/ In House Validation Report ERP Use Only - March 2015

Table 16. Results for testing a gliadin-contaminated stainless steel surface with an area of 10 x 10 cm for each swabbing experiment; 20 replicates per amount

µg gliadin/100 cm 2

result for each repeat

POD

- - - - - -

- - - - -

- - - - -

- - - - - - - -

-

-

0,00

0,00

- -

+

+ -

+ -

0,25

0,25

+ -

- -

-

-

-

+

+ + -

-

+ -

+ + -

0,50

0,25

- -

- -

- -

- -

-

- + + + + + + + + + + + + + + - - - - + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +

1,00

0,80

2,00

1,00

4,00

1,00

The results of these experiments are given in table 16. This is the only “matrix” were a higher degree of variation occurs. Besides no false-positives, the concentration range with negative and positive results was between 0.25 µg and 1.0 µg gliadin per 100 cm 2 surface. Starting with 2 µg gliadin/100 cm 2 , all results were positive. The testing of surfaces was the only case where two invalid results occurred (one at 0.5 µg/100 cm 2 ; one at 1 µg/100 cm 2 ). These two were tested again in a blind-coded manner.

AOAC Research Institute Expert Review Panel Use Only

Figure 4. Probabilities of detection (POD) for surface stainless steel (swabbing procedure); 20 replicates per gliadin amount.

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RIDA®QUICK Gliadin Validation report 2015-01-14