Review Book

5. Are there sufficient data points per product evaluated in accordance with AOAC requirements? Reviewer 1 YES Reviewer 2 YES Reviewer 3 YES Reviewer 4 YES Reviewer 5 YES

6. General Comments about the Method Scope/Applicability: Reviewer 1

Method applicable to a wide variety of foods and food-contact surfaces.

Reviewer 2 Reviewer 3 Reviewer 4

Appropriate

Scope and applicability match the PTM and MLV validation studies. Red page number (manuscript page number) 1.163(2)-Table 1-add sponge to Stainless steel surface, add swab to Polystyrene surface, add “Flavored” to Ranch seasoning. 2. 164(3)-add city, state to ATCC 3. 164(3)-how was the NFDM inoculated, and how mixed (manually?) 4.165(4)-Test Portion Analysis, 1st paragraph-“Out of 11 collaborators…” - probably should add “submitting data” or something, since there were actually 12 laboratories participating and abstract says “14 participants” 5. 167(6)-add “onion” to honey mustard seasoning 6. 167-168 (6-7)-please compare with IFU and match items/wording. For example, “sample boiling tubes” are (i)-Sample Tubes-12 mm external diameter and 75 mm height. 7. 168(7)-Letheen Broth and also could use HiCap Buffer 8. 169(8)-D(a)(a)-second (a) not necessary. Add “sample” to “To each 375 g, homogenize…” 9. 169(8)-D(a)(a)-add +/- 1 degrees 10. 170(9)-check (a)1. With IFU. IFU says 1-2 ml, here it says 1mL, add “to sample boiling tube”, “fatty matter” not in IFU. 11. 172(11)-please compare to IFU-some steps are left out before Stop Solution. 12. 172(11)-6)a. IFU says USDA/FSIS MLG 4.09, here it is 4.10 13. 173(12)-d (If no typical colonies are observed….”-check this with the IFU and the FDA procedure. 14. 175-176(14-15)-Discussion-there should be something about the use of DS2 and manual method. 15. 176(15)-Recommendations-add “onion” to honey mustard seasoning. 16. 177(16)-(4)-update OMA to 2019, 21st Ed. 17. 178(17)-Table 2-description of Collaborator 2 is not he same as in the text of the manuscript (voluntarily withdrew). The Solus One Salmonella Assay is a rapid and specific method.

Reviewer 5

7. Pros/Strengths of the Manuscript: Reviewer 1 Clearly presented. Reviewer 2 Clearly written Reviewer 3

Very well written. I wish that all of these manuscripts had a PTM data table in the Introduction.

Reviewer 4 Reviewer 5

International participants were used.

The Solus One Salmonella Assay is a rapid and specific method.