Review Book

Evaluation of the GENE-UP ® Salmonella Method for the Detection of Salmonella Species in a Broad Range of Foods and Select Environmental Surfaces: Collaborative Study

Ronald Johnson 1 , John Mills & Nikki Taylor

bioMérieux, Inc. 595 Anglum Road, Hazelwood, MO, 63042 USA Patrick M. Bird AOAC INTERNATIONAL, 2275 Research Blvd. #300, Rockville, MD, 20850 USA

Background : The GENE-UP ® Salmonella (SLM) assay ( Performance Tested Method SM 121802) is a PCR detection method that utilizes Fluorescence Resonance Energy Transfer hybridization probes for the rapid detection of Salmonella species in foods and on environmental surfaces. Objective : The purpose of this validation was to evaluate the method’s interlaboratory performance for submission to AOAC INTERNATIONAL for adoption as First Action Official Method of Analysis SM . Method : The GENE-UP ® method was evaluated in a multi-laboratory study using unpaired test portions for one food matrix, raw ground beef (80% lean). The candidate method was compared to the USDA FSIS MLG 4.10 reference method. An alternative confirmation procedure, using ASAP™ and CHROMID ® Salmonella chromogenic agars, was included in the validation study. Fifteen collaborators from 14 laboratories throughout the

1 Corresponding author. Ron.Johnson@biomerieux.com

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