Review Book

Water. (b) Mix and incubate at 42 ± 1°C for 18 ‑ 24 h. (c) Dilute 1/100 of the culture in BPW in order to obtain a suspension containing approximately 10 6 cells/mL of the strain. (d) Follow the protocol from the SAMPLE LYSIS section steps to CONFIRMATION OF POSITIVE RESULTS sections. (e) Check that the results obtained correspond to the characteristics of the tested strains.

K. Limitations of the Method

The GENE ‑ UP ® Salmonella (SLM) kit has been evaluated on a large number of matrices. However, given the wide variety of products and manufacturing procedures, it is recommended to check that the composition of the matrices tested does not affect the reliability of GENE ‑ UP ® results.

Results of Collaborative Study

The collaborative study involved a method comparison evaluation of the candidate method to the MLG 4.10 reference method for raw ground beef (80% lean). A total of 15 collaborators from 14 sites throughout the United States participated in this study. Each participant analyzed 36 unpaired test portions for the candidate method and the MLG reference method: 12 inoculated with a high level of Salmonella , 12 inoculated with a low level of Salmonella , and 12 non- inoculated controls. Of the 15 collaborators, 11 were included in the final statistical analysis. One laboratory was not able to complete testing and did not submit results. One laboratory did not perform the traditional confirmation procedure and were therefore excluded from the

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