Review Book

statistical analysis. Two laboratories (1 and 10) recovered the target analyte in their non- inoculated control samples and their data was not included in the statistical analysis. Tables 2020.1A and 1B summarize the results and POD statistical analysis of the collaborative study. Detailed results for each collaborator are presented in Table 2020.2A and 2020.2B. The individual collaborator test portion results are presented in Tables 1 and 2 of the Supplementary Materials. The concentration of Salmonella , and 95% confidence interval, in each inoculation level was determined on the day of initiation of analysis by the coordinating laboratory: 0.49 CFU/test portion (0.37, 0.63) for the low inoculum level and 3.43 CFU/test portion (2.72, 4.88) for the high inoculum level. The results of the APC analysis produced a range of 8.1 x 10 5 to 2.5 x 10 7 CFU/g for an average of 6.6 x 10 6 CFU/g. Complete APC results are presented in Table 3 of the Supplementary Materials. Temperature records obtained during the shipment of samples are presented in Table 4 of the Supplementary Materials. For the low inoculation level, 51 out of 132 test portions (POD CP of 0.39) were reported as presumptive positive by the candidate method with 50 out of 132 test portions (POD CC of 0.38) confirming positive. For the candidate method true positives, 49 out of 132 test portions (POD C of 0.37) were both presumptive positive and confirmed positive. For the reference method, 51 out of 132 test portions were reported as positive (POD R of 0.0.39). A dLPOD C value of -0.02 with 95% confidence interval of (-0.15, 0.12) was obtained between the candidate and reference method, indicating no statistically significant difference was found by the study. A dLPOD CP value of 0.01 with 95% confidence intervals of (-0.03, 0.04) was obtained between presumptive and confirmed results indicating no statistically significant difference was found by the study. Raw Ground Beef (80% lean) GENE-UP Salmonella with Alternative Confirmation

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