Review Book

Table 2020.2B: Comparative results for the detection of Salmonella in raw ground beef (80% lean) test portions by the GENE-UP ® Salmonella (SLM) 1 method with traditional confirmation vs. MLG 4.10 reference method in a collaborative study (non-inoculated control) 2

Candidate presumptive (CP)

Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

C vs. R CP vs. CC

Statistic

Matrix

Laboratory

POD (CP) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00 0.00

POD (CC) 0.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

POD (C) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

POD (R) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00 0.00

dLPOD (C,R)

dLPOD (CP,CC)

N

X

N

X

N

X

N

X

1 a

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 1 0 0 0

12 12 12 12 12 12 12 12 12 12 12 12 12

2 0 0 0 0 0 0 0 0 0 0 0 0

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 0 0 0 0

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 1 0 0 0

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.08 0.00 0.00 0.00

-0.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00 0.00

2 3 4 5 6 7 8 9

Raw ground beef (80% lean)

10 a

11 12 13

Estimate

All

132

0

0.00 0.00 0.03

132

0

0.00 0.00 0.03

132

0

0.00 0.00 0.03

132

0

0.00 0.00 0.03

0.00 -0.03 0.03 0.00 0.00 0.00 N/A

0.00 -0.02 0.02 0.00 0.00 0.00

LCL UCL

s r s L s R

b

c d

SE e

0.01 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e Standard Error

3 4 5 6

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit

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