Review Book
Table 2020.2B (cont’d.): Comparative results for the detection of Salmonella in raw ground beef (80% lean) test portions by the GENE-UP ® Salmonella 1 (SLM) method with traditional confirmation vs. MLG 4.10 reference method in a collaborative study (low inoculum level) 2
Candidate presumptive (CP)
Candidate confirmed (CC)
Candidate result (C)
Reference method (R)
C vs. R CP vs. CC
Statistic
Matrix
Laboratory
POD (CP) 0.33 0.42 0.42 0.50 0.42 0.33 0.33 0.25 0.42 0.25 0.33 0.50 0.33
POD (CC) 0.42 0.33 0.42 0.58 0.50 0.33 0.33 0.25 0.42 0.33 0.33 0.50 0.25
POD (C) 0.25 0.33 0.42 0.50 0.42 0.33 0.33 0.25 0.42 0.08 0.33 0.50 0.25
POD (R) 0.75 0.67 0.50 0.25 0.42 0.33 0.17 0.50 0.33 0.00 0.25 0.67 0.17
dLPOD (C,R)
dLPOD (CP,CC)
N
X
N
X
N
X
N
X
1 a
12 12 12 12 12 12 12 12 12 12 12 12 12
4 5 5 6 5 4 4 3 5 3 4 6 4
12 12 12 12 12 12 12 12 12 12 12 12 12
5 4 5 7 6 4 4 3 5 4 4 6 3
12 12 12 12 12 12 12 12 12 12 12 12 12
3 4 5 6 5 4 4 3 5 1 4 6 3
12 12 12 12 12 12 12 12 12 12 12 12 12
9 8 6 3 5 4 2 6 4 0 3 8 2
-0.50 -0.34 -0.08 0.25 0.00 0.00 0.16 -0.25 0.09 0.08 0.08 -0.17 0.08 -0.02 -0.15 0.12 0.50 0.00 0.50 N/A
-0.09 0.09 0.00 -0.08 -0.08 0.00 0.00 0.00 0.00 0.17 0.00 0.00 0.08 0.00 -0.04 0.04 0.50 0.00 0.50
2 3 4 5 6 7 8 9
Raw ground beef (80% lean)
10 a
MPN/ Test Portion
11 12 13
Estimate
All
132
51
0.39 0.33 0.44
132
51
0.39 0.31 0.46
132
49
0.37 0.31 0.43
132
51
0.39 0.27 0.51
0.49 0.37 0.63
LCL UCL
s r s L s R
b
c d
SE e
0.02 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e Standard Error
3 4 5 6
N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
37
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