Review Book

AOAC ERP Method Review Form

Submission Date

2020-03-03 06:43:20

Method Title:

Gene-UP Salmonella method for the detection of Salmonella in a broad range of foods

Expert Reviewer Name

Thomas Hammack

E-mail Address

thomas.hammack@fda.hhs.gov

1. Is this method scientifically and technically sound?

Yes

2. Have sufficient controls been used, including those required to calculate the rate of false ‐ positive and false- negative results where appropriate? 3. Is sufficient information included for system suitability determination and product performance or acceptance testing? 4. Are the conclusions statements valid based upon data presented? 5. Do you agree that the evidence or data from this and previous studies support the proposed applicability statement? 6. Are there sufficient data points per product evaluated in accordance with AOAC requirements?

Yes

Yes

Yes

Yes

Yes

General Comments about the Method Scope/Applicability:

The scope and applicability look fine. I just have one comment. On page 19, of the OMA method, Section D.h., it is stated that the romaine lettuce should be homogenized. Is this correct? Was it homogenized in the SLV? If it was, then fine. If it was not, then this instruction should be revised to indicate that the lettuce should be soaked.

Pros/Strengths of the Manuscript:

Very well written manuscript.

Cons/Weaknesses of the Manuscript:

None observed.