Review Team (February 2016)

Amino-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Amino Acids in Infant Formula and Adult/Pediatric Formulas Ultra High Performance Liquid Chromatography room temperature and heat in a preheated water bath at 55 ˚C for 5 minutes. Final concentrations of calibration standards are 1.0, 5.0, 10.0, 25.0 and 50.0 μM respectively. 3) Preparation of calibration standards for Tryptophan (alkaline hydrolysis method): Table 4: Stock std. Conc. (mg/L) Vol. of stock Std. (mL) Makeup Volume (mL) Calibration std. Conc.(mg/L) 500.0 1.00 10.0 50.0 500.0 0.50 10.0 25.0 500.0 0.20 10.0 10.0 50.0 1.00 10.0 5.00 50.0 0.20 10.0 1.00 1.0 1.00 10.0 0.10 1.0 0.20 10.0 0.02

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