Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Thiamine

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

Method Blank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area) Blank (Peak Area)

265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2 265.2>122.2

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.82

15055.6 15618.9 14562.7 15713.6 13737.0 13549.6 15270.3 15585.5 13433.8 12834.9

373.6 373.6 373.6 373.6 373.6 373.6 373.6 373.6 373.6 373.6

2.48 2.39 2.57 2.38 2.72 2.76 2.45 2.40 2.78 2.91

1 2 3 4 5 6 7 8 9

10

Mean

Mean

2.583

RT Difference (criteria = ± 0.2 minute)

Concentration Analyte Retention Time (Minutes)

265.2>81.3

Ion Ratio % Relative Intensity

Ion Ratios

Overall

(μg/kg)

Area (Q1) 9029.51718 16489.33435 165868.5171 374491.9321 768752.4174 1952246.075

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

2561 4122

0.284 0.250 0.259 0.267 0.256 0.254

28.4 25.0 25.9 26.7 25.6 25.4

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

1.823 1.823 1.823 1.823 1.823 1.823 1.823 1.805 1.823 1.823 1.823 1.823 1.823

42924

100004 196894 495250

Mean

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

281221.43 287193.93 305591.61 304869.60 293676.06 287956.46

75498.00 76109.00 80795.00 80035.00 76317.00 74177.00

0.27 0.27 0.26 0.26 0.26 0.26

27 27 26 26 26 26

-0.018 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.26

26

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.18 0.34

Page 5

Upper limit

< 10