Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC-SPIFAN-2012-VITAMINS

Specificity

ValidationParameter

TestGroupName

Date

B-ComplexVitamins

01.01.2015

TestName

DataPathFile

Riboflavin

2015\January\Vitamins\01.01.2015

Matrix

Raw-DataFileRef.

MethodBlank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

ManjunathK/PrasadBKVR

Area%ofblank to lowestcalibrant (Criteria=<30%)

Lowestcalibrant concentrationug/l

SampleBlankNo.

RetentionTime (min)

m/z

Standard (PeakArea)

Blank (PeakArea)

377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

411.0 411.0 411.0 411.0 411.0 411.0 411.0 411.0 411.0 411.0

3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.94

4222.7 4220.2 4077.6 4147.9 4032.4 4038.2 4204.4 4272.9 4107.4 4115.9 Mean

9.73 9.74

1 2 3 4 5 6 7 8 9

10.08 9.91 10.19 10.18 9.78 9.62 10.01 9.99 9.922

10

Mean

RT Difference (criteria = ± 0.2 minute)

Analyte Retention Time (Minutes)

377.1>172.1

% Relative Intensity

Concentration

Ion Ratio

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

4348.308564 7748.884013 40826.16555 82574.58657 173711.2294 429749.2638

2375 4810

0.546 0.621 0.581 0.585 0.587 0.590

54.6 62.1 58.1 58.5 58.7 59.0

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

3.941 3.941 3.941 3.924 3.941 3.941 3.938 3.941 3.924 3.923 3.941 3.941 3.941

23723 48278 102048 253557

Mean

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

88826.34 86761.32 87018.09 87652.85 86242.42 87028.15

51682.00 51306.00 50127.00 51262.00 51809.00 50820.00

0.58 0.59 0.58 0.58 0.60 0.58

58 59 58 58 60 58

0.003 -0.015 -0.015 0.003 0.003 0.003

Relative intensity (%of basepeak)

Average ion ratio Average intensity

0.59

%Toleranceplusorminus

59

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.41 0.76

Page 6

Upper limit

<10