Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Method Blank Niacinamide

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area) Blank (Peak Area)

123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3 123.2>80.3

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.32 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315 2.315

14603.1 14530.7 13703.9 13923.9 14485.7 13793.1 14639.9 13597.7 14111.2 13738.9

3744.0 3744.0 3744.0 3744.0 3744.0 3744.0 3744.0 3744.0 3744.0 3744.0

25.64 25.77 27.32 26.89 25.85 27.14 25.57 27.53 26.53 27.25

1 2 3 4 5 6 7 8 9

10

Mean

Mean

26.550

RT Difference (criteria = ± 0.2 minute)

Concentration Analyte Retention Time (Minutes)

123.2>53.3

Ion Ratio % Relative Intensity

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

14568.43882 28956.77542 114474.3637 209845.4323 581016.6522 875832.1504

6188

0.425 0.383 0.440 0.418 0.287 0.421

42.5 38.3 44.0 41.8 28.7 42.1

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

11076 50365 87686

166838 368607

Mean

884894.43 899294.52 893797.19 909753.39 892082.67 919462.53

328272.00 330416.00 329408.00 330791.00 323780.00 332782.00

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

0.37 0.37 0.37 0.36 0.36 0.36

37 37 37 36 36 36

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.40

40

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.28 0.51

Upper limit

< 10

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