Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Method Blank Nicotinic acid

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1 124.2>80.1

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.87 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867 1.867

3069.0 3032.7 2779.1 3012.4 3343.1 3230.8 3161.2 2919.9 2933.1 3066.1 Mean

653.0 653.0 653.0 653.0 653.0 653.0 653.0 653.0 653.0 653.0

21.28 21.53 23.50 21.68 19.53 20.21 20.66 22.36 22.26 21.30

1 2 3 4 5 6 7 8 9

10

Mean

21.431

RT Difference (criteria = ± 0.2 minute)

% Relative Intensity

Concentration Analyte Retention Time (Minutes)

124.2 > 78.1

Ion Ratio

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

3013.099027 5670.810404 26674.59717 50125.53391 92926.1074 186487.7079

2907 5539

0.965 0.977 0.920 0.902 0.915 0.873

96.5 97.7 92.0 90.2 91.5 87.3

24536 45199 85044 162873

Mean

21884.60 21997.95 22494.65 22371.28 22131.94 21606.36

20694.00 20833.00 21173.00 20886.00 20807.00 20389.00

Eezee paste-1 Eezee paste-2 Eezee paste-3 Eezee paste-4 Eezee paste-5 Eezee paste-6

0.95 0.95 0.94 0.93 0.94 0.94

95 95 94 93 94 94

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.93

93

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.65 1.20

Upper limit

< 10

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