Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Pantothenic acid

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

Method Blank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0 220.1>90.0

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

2682.0 2682.0 2682.0 2682.0 2682.0 2682.0 2682.0 2682.0 2682.0 2682.0

2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.84 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843

9257.6 8551.1 8818.9 8773.9 9107.2 9144.9 9323.9 8828.1 8738.3 8949.5 Mean

28.97 31.36 30.41 30.57 29.45 29.33 28.76 30.38 30.69 29.97

1 2 3 4 5 6 7 8 9

10

Mean

29.990

RT Difference (criteria = ± 0.2 minute)

% Relative Intensity

Concentration Analyte Retention Time (Minutes)

220.1>202.1

Ion Ratio

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

9491.885498 18222.57862 93229.19028 181246.3689 355269.3599 700583.3038

3459 6438

0.364 0.353 0.356 0.363 0.367 0.359

36.4 35.3 35.6 36.3 36.7 35.9

33162 65844 130367 251451

Mean

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

587092.12 596359.89 591637.57 605049.95 592662.67 588123.52

212140.00 215759.00 209899.00 217663.00 214701.00 211619.00

0.36 0.36 0.35 0.36 0.36 0.36

36 36 35 36 36 36

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.36

36

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.25 0.47

Upper limit

< 10

Page 9