Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Pyridoxin

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

Method Blank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1 170.2>152.1

5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

695.0 695.0 695.0 695.0 695.0 695.0 695.0 695.0 695.0 695.0

2.097 2.097 2.097 2.097 2.097 2.097 2.097 2.097 2.097 2.097 2.10

46838.4 46494.7 47375.1 45064.5 47882.5 45994.3 45994.5 46518.0 47576.3 47071.2

1.48 1.49 1.47 1.54 1.45 1.51 1.51 1.49 1.46 1.48

1 2 3 4 5 6 7 8 9

10

Mean

Mean

1.489

RT Difference (criteria = ± 0.2 minute)

% Relative Intensity

Concentration Analyte Retention Time (Minutes)

170.2>134.1

Ion Ratio

Ion Ratios

Overall

(μg/kg)

Area (Q1) 52068.7609 93709.7676 445905.4511 801082.5817 1420134.093 2771730.763

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

37624 68929

0.723 0.736 0.748 0.761 0.770 0.768 0.784 0.789 0.798 0.786 0.802 0.78

72.3 73.6 74.8 76.1 77.0 76.8 78.0 78.4 78.9 79.8 78.6 80.2

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843 2.843

333530 609512 1093000 2129000

Mean

552992.20 567524.26 556656.83 559318.84 569052.39 554270.79

431534.00 444719.00 439467.00 446583.00 447307.00 444354.00

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.76

76

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.53 0.99

Upper limit

< 10

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