Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Biotin

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

Method Blank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3

2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0

3.838 3.838 3.856 3.856 3.838 3.856 3.856 3.856 3.856 3.856 3.85 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803

18810.3 18322.9 18722.6 18757.1 18524.8 18848.9 18339.0 18615.1 18471.9 18492.6

5425.0 5659.0 5565.0 5539.0 5586.0 5432.0 5407.0 5728.0 5571.0 5458.0

28.84 30.88 29.72 29.53 30.15 28.82 29.48 30.77 30.16 29.51

1 2 3 4 5 6 7 8 9

10

Mean

Mean

29.788

RT Difference (criteria = ± 0.2 minute)

Concentration Analyte Retention Time (Minutes)

245.2>97.1

Ion Ratio

% Relative Intensity

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

21217.74585 37598.44009 167952.8837 317423.9198 604434.3372 1175276.621

4518 7591

0.213 0.202 0.196 0.200 0.198 0.199 0.203 0.200 0.198 0.203 0.199 0.198

21.3 20.2 19.6 20.0 19.8 19.9 20.3 20.0 19.8 20.3 19.9 19.8

32877 63463 119692 233339 7913.00 8012.00 7777.00 7997.00 7931.00 7830.00

Mean

39001.20 40053.53 39267.12 39344.38 39895.70 39580.06

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.20

20

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.14 0.26

Upper limit

< 10

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