Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Specificity

Validation Parameter

Test Group Name

Date

B-Complex Vitamins

01.01.2015

Test Name

Data Path File

Folic acid

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

Method Blank

TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS

Manjunath K/Prasad BKVR

Area % of blank to lowest calibrant (Criteria = <30%)

Lowest calibrant concentration ug/l

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

442.2>295 442.2>295 442.2>295 442.2>295 442.2>295 442.2>295 442.2>295 442.2>295 442.2>295 442.2>295

2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0

3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.33 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333 3.333

1323.4 1359.8 1363.8 1268.4 1310.4 1276.8 1325.8 1342.0 1518.9 1359.0 Mean

187.0 187.0 187.0 187.0 187.0 187.0 187.0 187.0 187.0 187.0

14.13 13.75 13.71 14.74 14.27 14.65 14.10 13.93 12.31 13.76

1 2 3 4 5 6 7 8 9

10

Mean

13.937

RT Difference (criteria = ± 0.2 minute)

% Relative Intensity

Concentration Analyte Retention Time (Minutes)

442.2>176.0

Ion Ratio

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed Confirmed

Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass Pass

Calibration standard 1 Calibration standard 2 Calibration standard 3 Calibration standard 4 Calibration standard 5 Calibration standard 6

2975.057627 5939.641659 30455.02702 63141.05518 135368.6573 365563.4524

907

0.305 0.317 0.307 0.308 0.310 0.309 0.327 0.303 0.324 0.299 0.299 0.340

30.5 31.7 30.7 30.8 31.0 30.9 32.7 30.3 32.4 29.9 29.9 34.0

1883 9337

19441 41994 112807 2564.00 2512.00 2570.00 2385.00 2435.00 2559.00

Mean

7844.17 8277.19 7934.49 7983.66 8153.55 7529.87

SRM 1849a-1 SRM 1849a-2 SRM 1849a-3 SRM 1849a-4 SRM 1849a-5 SRM 1849a-6

0.000 0.000 0.000 0.000 0.000 0.000

Relative intensity (% of base peak)

% Tolerance plus or minus

Average ion ratio Average intensity

0.31

31

>50

30 30 30 30

30.00

>20-50 >10-20

Tolerance (%) Lower limit

0.22 0.40

Upper limit

< 10

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