Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

Test Group Name

B-complex Vitamins Riboflavin Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

0.00 50000.00 100000.00 150000.00 200000.00 250000.00 300000.00 350000.00 400000.00 450000.00

Calibration Standards

Concentration (ug/kg)

Area-Analyte

y = 801.89x - 3934.8 R² = 0.9997

5.00

3037.84

Solvent Standard Level-1

10.00

6191.24

Solvent Standard Level-2

50.00

34554.87

Solvent Standard Level-3

100.00

71591.71

Solvent Standard Level-4

300.00

237068.98

Solvent Standard Level-5

500.00

397766.42

Solvent Standard Level-6

0.00

100.00 200.00 300.00 400.00 500.00 600.00

R 2 ≥ 0.99

Criteria

Conclusion

Complies

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