Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

B-complex Vitamins Niacinamide Aqueous

Test Group Name

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

Calibration Standards

Concentration (ug/kg)

Area-Analyte

Solvent Standard Level-1

5.00

13753.35

0.00 100000.00 200000.00 300000.00 400000.00 500000.00 600000.00 700000.00 800000.00 900000.00 1000000.00

y = 1800x + 19977 R² = 0.9976

Solvent Standard Level-2

10.00

25632.40

Solvent Standard Level-3

50.00

114620.22

Solvent Standard Level-4

100.00

216982.92

Solvent Standard Level-5

300.00

583442.21

Solvent Standard Level-6

500.00

902435.56

R 2 ≥ 0.99 Complies

Criteria

0.00

100.00 200.00 300.00 400.00 500.00 600.00

Conclusion

Page no. 15