Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

Test Group Name

B-complex Vitamins Pantothenic acid Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

0.00 100000.00 200000.00 300000.00 400000.00 500000.00 600000.00 700000.00

Calibration Standards

Concentration (ug/kg)

Area-Analyte

y = 1269.4x + 10759 R² = 0.9957

Solvent Standard Level-1 Solvent Standard Level-2 Solvent Standard Level-3 Solvent Standard Level-4 Solvent Standard Level-5 Solvent Standard Level-6

5.00 20.00 50.00 100.00 400.00 500.00

6997.95 29377.70 75320.57 150423.73 545240.86 621801.71

R 2 ≥ 0.99

Criteria

0.00

100.00 200.00 300.00 400.00 500.00 600.00

Conclusion

Complies

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