Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

Test Group Name

B-complex Vitamins Pyridoxin Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

2500000.00

Calibration Standards

Concentration (ug/kg)

Area-Analyte 42920.38 86685.62 411233.87 747190.82 1356939.66 1895074.96

y = 6283.8x + 60220 R² = 0.9954

2000000.00

Solvent Standard Level-1 Solvent Standard Level-2 Solvent Standard Level-3 Solvent Standard Level-4 Solvent Standard Level-5 Solvent Standard Level-6

5.00 10.00 50.00 100.00 200.00 300.00

1500000.00

1000000.00

500000.00

0.00

0.00 50.00 100.00150.00200.00250.00300.00350.00

R 2 ≥ 0.99

Criteria

Conclusion

Complies

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