Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Parameter

Test Group Name

B-complex Vitamins Biotin Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

With Multilevel Calibration

0.00 100000.00 200000.00 300000.00 400000.00 500000.00 600000.00 700000.00

Calibration Standards

Concentration (ug/kg)

Area-Analyte

y = 2847.5x + 7238.9 R² = 0.9996

Solvent Standard Level-1 Solvent Standard Level-2 Solvent Standard Level-3 Solvent Standard Level-4 Solvent Standard Level-5 Solvent Standard Level-6

2.00 10.00 20.00 50.00 100.00 200.00

9670.47 32379.76 64275.76 153756.67 298417.19 572682.07

R 2 ≥ 0.99

Criteria

0.00

50.00

100.00

150.00

200.00

250.00

Conclusion

Complies

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