Review Team (February 2016)
BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE
AOAC- SPIFAN-2012-VITAMINS
Validation Parameter
Limit of Quantification
Test Group Name
Date
2015\January\Vitamins\01.01.2015 01.01.2015
B-Complex vitamins Biotin
Test Name
Data Path File
Matrix
Raw-Data File Ref. CRM Data File Ref.
TUV/BLR/F-360 TUV/BLR/F-99
Aqueous
Limit
Equipment Test Method
Analyst
Prasad BKVR/Kesav Kumar N Manjunath K/Prasad BKVR
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Operator
Repeatability at LOQ 2 ug/l standard Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
Caluclation
245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3 245.2>227.3
3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803 3.803
1.874 1.933 1.950 1.889 1.933 1.782 1.897 1.857 1.914 1.932
1 2 3 4 5 6 7 8 9
Mean (μg/l)
1.90
SD
0.0497
LOD (Mean +3 sigma) LOQ (Mean + 10sigma)
2.05 2.39 2.62
RSD %
2
Standard Conc. (μg/l)
94.8
Accuracy %
Accuracy = 70 - 125 %
Criteria
10
RSD% = <15%
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