Review Team (February 2016)

Amino-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Sample Name: Amino acids Mix-5uM

===================================================================== Acq. Operator : Seq. Line : 6 Acq. Instrument : UPLC-DAD Location : P1-D-03 Injection Date : 7/6/2015 10:06:50 PM Inj : 1 Inj Volume : 1.000 μl Acq. Method : D:\UPLC\DATA\COE\2015\JULY2015\04AMINOACIDS-VAL-2\04AMINOACIDS-VAL2 2015-07 -06 20-53-07\V-AMINOACIDS-1.M Last changed : 7/6/2015 8:53:08 PM Analysis Method : D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\V-AMINOACIDS-1.M (Sequence Method) Last changed : 8/1/2015 2:35:37 PM (modified after loading) Additional Info : Peak(s) manually integrated

DAD1 A, Sig=260,4 Ref=off (D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\_006_AMINO ACIDS MIX-5UM-SS1.D)

mAU

35

30

7.525

25

20

15

Area: 16.6504 6.796 Area: 27.5474 6.878 Area: 19.1716 6.988 7.182 7.361

10

8.632

8.372

8.489

Area: 18.0406 5.860

5

5.248

4.863

4.450

3.475

3.604

3.937

3.298

3.030

2.295

0

2

4

6

8

10

min

===================================================================== Internal Standard Report with Performance ===================================================================== Calib. Data Modified : Saturday, August 01, 20152:35:11 PM Multiplier : 1.0000 Dilution : 1.0000 Do not use Multiplier & Dilution Factor with ISTDs

Signal 1: DAD1 A, Sig=260,4 Ref=off

RetTime k' Sig Amount Symm. Width Plates Resol Name [min] [μM] [min] ution -------|------|---|----------||-----|-------|-------|-----|---------- 2.295 - 1 0.00000 0.79 0.1067 2564 - Histidine 3.030 - 1 0.00000 0.71 0.0858 6894 4.49 Taurine 3.298 - 1 0.00000 0.76 0.0779 9937 1.92 Serine 3.475 - 1 0.00000 0.83 0.0702 13563 1.40 Argenine 3.604 - 1 0.00000 0.75 0.0793 11437 1.01 Glycine

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UPLC-DAD 8/1/2015 2:35:40 PM