Review Team (February 2016)

Amino-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Sample Name: Amino acids Mix-5uM

===================================================================== Acq. Operator : Seq. Line : 6 Acq. Instrument : UPLC-DAD Location : P1-D-03 Injection Date : 7/6/2015 10:06:50 PM Inj : 1 Inj Volume : 1.000 μl Acq. Method : D:\UPLC\DATA\COE\2015\JULY2015\04AMINOACIDS-VAL-2\04AMINOACIDS-VAL2 2015-07 -06 20-53-07\V-AMINOACIDS-1.M Last changed : 7/6/2015 8:53:08 PM Analysis Method : D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\V-AMINOACIDS-1.M (Sequence Method) Last changed : 8/1/2015 2:39:21 PM (modified after loading) Additional Info : Peak(s) manually integrated

DAD1 A, Sig=260,4 Ref=off (D:\UPLC\DATA\COE\2015\JULY2015\AA-VAL1\_006_AMINO ACIDS MIX-5UM-SS1.D)

mAU

35

30

7.525

25

20

15

Area: 16.6504 6.796 Area: 27.5474 6.878 Area: 19.1716 6.988 7.182 7.361

10

8.632

8.372

8.489

Area: 18.0406 5.860

5

5.248

4.863

4.450

3.475

3.604

3.937

3.298

3.030

2.295

0

2

4

6

8

10

min

===================================================================== Internal Standard Report with Performance and Noise ===================================================================== Calib. Data Modified : Saturday, August 01, 20152:35:11 PM Multiplier : 1.0000 Dilution : 1.0000 Do not use Multiplier & Dilution Factor with ISTDs

Signal 1: DAD1 A, Sig=260,4 Ref=off

Noise determination:

Time range Noise Noise Noise from | to | (6*SD) | (PtoP) | (ASTM) | Wander | Drift [min] | [min] | [mAU] | [mAU] | [mAU] | [mAU] | [mAU/h] -------|-------|---------|---------|---------|---------|--------- 4.100 4.250 6.336e-2 3.486e-2 - - 36.185 6.000 6.500 0.1294 8.940e-2 - - 45.421 7.700 8.200 0.1290 8.685e-2 - - 31.809

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UPLC-DAD 8/1/2015 2:39:24 PM