Review Team (February 2016)

Fonterra Co-operative Group VitD-18 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix A: Data—Linearity Table A1: Data vitamin D 2

linearity experiment 1

Concentration (1) NLD 2 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.3 1.5 7.6

0.04 0.18 0.90 1.80 4.49 7.18

0.03 0.17 0.81 1.64 4.06 6.45

0.03 0.18 0.89 1.81 4.50 7.17

-29.0

-0.7 -0.9

15.1 37.9 60.6

1.0 0.3

-0.2

Regression Equation

y = 0.8987x + 0.0105

(1) Concentration of non-labelled vitamin D 2 in calibration standards (2) Ratio of concentration of non-labelled vitamin D 2 to isotope-labelled vitamin D 2 in calibration standards

Table A2: Data vitamin D 2

linearity experiment 2

Concentration (1) NLD 2 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.3 1.5 7.6

0.04 0.18 0.90 1.79 4.48 7.18

0.03 0.15 0.78 1.58 3.90 6.17

0.03 0.16 0.89 1.82 4.51 7.15

-30.1

-8.5 -0.4

15.1 37.8 60.5

1.3 0.6

-0.3

Regression Equation

y = 0.8609x + 0.0117

(1) Concentration of non-labelled vitamin D 2 in calibration standards (2) Ratio of concentration of non-labelled vitamin D 2 to isotope-labelled vitamin D 2 in calibration standards

Table A3: Data vitamin D 2

linearity experiment 3

Concentration (1) NLD 2 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.3 1.4 7.2

0.03 0.17 0.86 1.71 4.28 6.84

0.03 0.15 0.74 1.50 3.77 5.88

0.02 0.16 0.84 1.72 4.35 6.80

-34.4

-5.8 -1.8

14.4 36.1 57.8

0.6 1.7

-0.7

Regression Equation

y = 0.8624x + 0.0137

(1) Concentration of non-labelled vitamin D 2 in calibration standards (2) Ratio of concentration of non-labelled vitamin D 2 to isotope-labelled vitamin D 2 in calibration standards

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