Review Team (February 2016)

Fonterra Co-operative Group VitD-18 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

Table A4: Data vitamin D 3

linearity experiment 1

Concentration (1) NLD 3 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.5 2.3

0.14 0.71 3.53 7.06

0.20 1.01 4.93 9.89

0.18 0.75 3.50 6.97

25.0

5.6

11.6 23.2 57.9 92.6

-1.0 -1.3

17.66 28.26

25.22 40.18

17.73 28.24

0.4

-0.1

Regression Equation

y = 1.4247x – 0.0479

(1) Concentration of non-labelled vitamin D 3 in calibration standards 2) Ratio of concentration of non-labelled vitamin D 3 to isotope-labelled vitamin D 3 in calibration standards

Table A5: Data vitamin D 3

linearity experiment 2

Concentration (1) NLD 3 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.5 2.3

0.14 0.70 3.52 7.04

0.21 0.96 5.19

0.22 0.71 3.45 6.67

55.8

0.4

11.5 23.1 57.7 92.3

-1.8 -5.2

10.13 27.92 42.65

17.59 28.14

18.25 27.83

3.8

-1.1

Regression Equation

y = 1.537x + -0.1227

(1) Concentration of non-labelled vitamin D 3 in calibration standards (2) Ratio of concentration of non-labelled vitamin D 3 to isotope-labelled vitamin D 3 in calibration standards

Table 7: Data vitamin D 3

linearity experiment 3

Concentration (1) NLD 3 (ng mL -1 )

Concentration Ratio (2)

Back-calculated Conc. Ratio

Variance (%)

Peak Area Ratio

0.5 2.3

0.14 0.71 3.56 7.12

0.20 0.97 4.69 9.76

0.26 0.80 3.44 7.04

79.4 11.9 -3.5 -1.3 -0.3

11.7 23.4 58.4 93.4

17.81 28.50

24.87 40.11

17.76 28.57

0.2

Regression Equation

y = 1.4096x – 0.1562

(1) Concentration of non-labelled vitamin D 3 in calibration standards (2) Ratio of concentration of non-labelled vitamin D 3 to isotope-labelled vitamin D 3 in calibration standards

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