SPADA Book - April 11, 2017

Technical Assessment: Sequencing Working Group  Discussion and analysis based on August 2016 National Academy of Sciences discussion regarding the potential for use of amplicon sequencing  Focused on technical assessment of metagenomic and amplicon sequencing • Document and/or leverage existing sequencing activities across multiple agencies and laboratories • Assess strengths and weaknesses of approaches

Membership — Sequencing Experts • Federal agencies

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• BioWatch laboratories • Other laboratory experts

 Meetings or teleconferences on a monthly or bi-weekly basis

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Informing the Sequencing Working Group Development of Program Requirements • “Value to stakeholder” sequencing discussion with local laboratory directors/designees • Identify level of characterization required to inform public health decisions • Develop stakeholder questionnaire • Effectiveness criteria • Suitability criteria • Feedback generated will inform the Program requirement development and discussions of the Sequencing Working Group

Evaluate targeted amplicon sequencing as well as metagenomics

Identify opportunities to leverage clinical and non-clinical applications; areas of overlap as well as separation

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