SPADA Book - April 11, 2017

Discussion

 How do the different agencies currently validate their sequencing methods?  Can we validate amplicon sequencing as well as metagenomic sequencing assays using the same set of standards? Or should there be different standards for each?  Would the model proposed by Ladner et al. work for organisms more complex than viruses? • Is it just a matter of scale? • Are there other considerations?

 Development of standards for testing: • Should they be developed internally?

• Is there a benefit to developing an overarching set of standards across organizations and sequencing efforts? If so, what are the challenges? What criteria/variables must be considered?

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