SPADA Book - April 11, 2017

Technical Suggestion

• 83‐86: the size of the eukaryotic genomes makes  using a 10X concentration of the genome difficult  as the quantity of DNA is huge.  Using a  concentration equivalent to the 10X of the  bacterial genome still provides a background but is  not so viscous.  Alternatively, you can place a  maximum DNA concentration on testing such as  100ug of background DNA.

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Proposed Change

• 83‐86:Organisms may be tested as pools  containing ten organisms each represented at 10  times the AMDL. Cell lines should be tested  individually at a DNA concentration equivalent to  10X AMDL.  These tests should be negative for the  target sequence.  The same pools can be spiked  with 2X the AMDL for the target organism, mixed  and tested. These tests should be positive for the  target in the high DNA background .

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