SPADA Book - April 11, 2017
Technical Suggestion
• 83‐86: the size of the eukaryotic genomes makes using a 10X concentration of the genome difficult as the quantity of DNA is huge. Using a concentration equivalent to the 10X of the bacterial genome still provides a background but is not so viscous. Alternatively, you can place a maximum DNA concentration on testing such as 100ug of background DNA.
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Proposed Change
• 83‐86:Organisms may be tested as pools containing ten organisms each represented at 10 times the AMDL. Cell lines should be tested individually at a DNA concentration equivalent to 10X AMDL. These tests should be negative for the target sequence. The same pools can be spiked with 2X the AMDL for the target organism, mixed and tested. These tests should be positive for the target in the high DNA background .
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