SPADA Book - April 11, 2017

DRAFT ENVIRONMENTAL ORGANISMS PANEL, VERSION 5

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Part 2:  Environmental Panel Organisms 

2.1  46 47 Airborne soil particles may constitute a significant challenge to assays for analysis of aerosol collection  48 filters and/or liquids.  Soils contain genomic materials or nucleic acid fragments of countless  49 archaebacterial, bacterial, and eukaryotic organisms.  Some of the more common soil organisms can be  50 anticipated.  Soils may also contain unanticipated inhibitors that interfere with sample processing and  51 detection.    52 53 Therefore, determining the effect of a variety of representative soils on an assay is an important first  54 step in establishing the specificity and robustness of an assay in the presence of interfering compounds.    55 56 To challenge assay specificity, use the assay to evaluate each regional soil type *† for positive responses.     57 58 There is no consensus on a course of action if a positive response is detected.  Therefore, it is incumbent  59 on the method developer to determine the appropriate course of action if a positive response is  60 detected.    61 62 63 64 65 66 67 68 † See section 2.2 “Bioinformatics Analysis” on probing all available data bases including those that  69 contain soil metagenome sequences generated from specific regions of operations (if available) for  70 In‐Silico Analysis and further validation of the signature sequences.  Soil Testing 

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