SPADA Book - April 11, 2017

The yeast could “serve as a viable resource for the first phase of training programs” and “be used periodically as a ‘confidence checker’ to allow first responders, under controlled conditions, to review or practice their skills.” – APHL Public Health Preparedness and Response (PHPR) Committee Stakeholder Input on the Use of the Yeast Yeast can “provide responders the ability to exercise and evaluate sample collection… with material that safeguards against the potential of cross contamination” and “be used as a competency assessment tool to evaluate the process of sample collection/submission.” – CPT Bryon Marsh (ret), 4th CST

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Prototype Batch of Lyophilized Yeast • Homogeneity – Determine vial-to-vial variability in terms of total cells per vial and colony forming units (CFUs) per vial • Stability – Assess total and viable cells per vial for real-time and accelerated test conditions; Also evaluate DNA stability/integrity

Viability (live cells) Plate counting Live/dead staining

Quantity (total cells) Coulter counter Hemocytometer

Insert stability qPCR WGS

Reference value

• Fitness for purpose – Evaluate suitability for end-user requirements and applications

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