SPADA Book - April 11, 2017

Yeast Incorporation into the Exercise

Day 2

Day 1

Day 3

On-site yeast prep by CSTs

Team assembly and JIT training

Sample Placement

Plume model

Sample collection

Decon and chain of custody

On-site DNA extraction and qPCR

On-site results

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Next – Yeast as a Surrogate Powder

Potential RM format Powder

Example workflow

Objective: Establish a protocol for first responder training in sample collection and field assessment using yeast as a surrogate powder

Sampling

New interlab

Spiked swabs

Processing

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Part 1: Confirm the limit of

DNA extraction

detection for yeast in PHLs

Cell suspension

Previous interlab

Part 2: Evaluate a protocol for sampling and detection of yeast powder

Genomic DNA

Detection

Data interpretation & reporting

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