SPADA Book - April 11, 2017

Known Mixtures of DNA or Cells are Needed • Technologies are advancing from targeted detection of one or a few agents to untargeted detection of multiple unknowns • “Ground truth” samples are needed for these technologies to realize their potential to revolutionize biological detection

“Microbial standard reference materials…will be a valuable tool for use in this evaluation.” devices as systems, including all of the components necessary to generate a result.” “FDA intends to regulate Infectious Disease NGS Dx

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Mixtures of Microbial DNA or Whole Cells • Goal: Develop (or enable development of) control and reference materials based on DNA or cell mixtures to increase confidence in biological detection technologies

Organisms in matrix

Data

Results

Environment

DNA

Sample

Extract

Sequence

Informatics

Cell Mixture

DNA Mixture

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