SPADA Book - April 11, 2017

Approach – Build on Existing NIST Efforts

SRM 2374 for RNA spike-in mixtures

RM 8375: DNA from 4 microbes

Toward a mixed DNA material

Bioinformatic pipelines to characterize DNA

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Initial efforts toward a mixed pathogen DNA RM

Cell quantification methods for yeast

NIST Candidate RM for whole yeast cells

Toward a mixed cell material

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Standards for Pathogen Identification via Next- Generation Sequencing (SPIN) Workshop October 20-21, 2014 at NIST • Identify measurement challenges and potential solutions associated with using NGS for pathogen identification • On the wish list - DNA, RNA, or cell mixtures at known ratios - Guidelines to develop in-house mixtures

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