SPADA Book - April 11, 2017

NIST-FDA Workshop: Standards for Pathogen Detection via Next-Generation Sequencing October 27-28, 2015 at NIST

Action item: Develop a mixed pathogen DNA material

#NISTpathogen

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Prototype Mixed Pathogen DNA Material

Tube Rack – 25 Tubes

• Genome assembly • Base level purity (e.g. rare variants) • Genomic contaminants • Quantity (~ 50-100 ng/μL), to infer genomes per μL • Stability Each tube will contain a single genome and will be characterized:

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11 12 13 14 15

16 17 18 19 20

21 22 23 24 25

Tubes 1-24: Clinically-relevant microbial pathogens Tube 25: Human DNA as “background”

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